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美國戴安 P/N: 037023Dionex AminoPac PA1 Amino Acid Column 生物色譜柱

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產品名稱: 美國戴安 P/N: 037023Dionex AminoPac PA1 Amino Acid Column 生物色譜柱
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【簡單介紹】戴安公司生產的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術優勢和方法已廣泛地應用于各個領域,其市場占有率達80%。全球與離子色譜相關的文章有90%均出自戴安公司生產的離子色譜儀。   
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美國戴安 P/N: 037023Dionex AminoPac PA1 Amino Acid Column 生物色譜柱  的詳細介紹

AminoPac PA1 Amino Acid Column

For Specialized Amino Acid Applications with Postcolumn Derivatization

The AminoPac??PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead? particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

  • Resolves phosphorylated and other amino acids by anion-exchange chromatography
  • Separates acid-labile amino acids under basic pH conditions
  • Separates strongly acidic amino acids
  • Requires OPA or ninhydrin postcolumn derivatization

The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization? Specifications
DimensionsAminoPac PA1 Analytical Column:
4?×?250 mm
AminoPac PA1 Guard Column:
4 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)
Recommended operating temperatureAmbient
Typical operating conditions1.0 mL/min gradient
Recommended flow rate: 1.0 mL/min
Mobile phase compatibilityCompatible with up to 5% common organic solvents.
Recommended eluents: hydroxide, borate, and acetate.
Resin composition10-μm-diameter, pellicular substrate (2% cross-linked)
agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
Anion-exchange capacityN/A
Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
Analytical Columns
AminoPac PA1 Analytical Column (4 x 250 mm)037022
Guard Columns
AminoPac PA1 Guard Column (4 x 50 mm)037023
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