DNAPac PA100 Nucleic Acid Column
For n, n-1 Resolution of Single-Stranded Oligonucleotides
The DNAPac??PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.
- Capable of n, n-1 resolution for oligonucleotides
- Resolves oligonucleotides with secondary structures
- Compatible with solvent, high pH, and high temperatures
- Analyzes phosphorothioate-based clinical samples
- Provides easy scale-up
The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.
Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.
Separates RNA / Double-Stranded DNA
The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.
Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.
| DNAPac??PA100 for n, n - 1 Resolution of Single-Stranded Oligonucleotides?Specifications |
|---|
| Temperature range | 4–90 °C |
| Typical operating conditions | 2400 psi at 1.0 mL/min (analytical and guard columns)
Recommended flow rate: 1.0 mL/min |
| Mobile phase compatibility | pH 2–12.5; 100% compatible with common organic solvents.
Ionic form eluents: chloride, acetate, or bromide in lithium, sodium, or ammonium forms. |
| Resin composition | 13 ?m-diameter nonporous substrate (55% cross-linking) agglomerated with a 100 nm MicroBead alkyl quaternary ammonium functionalized latex (5%?cross-linked). |
| Anion-exchange capacity | ~40 ?eq/column (4?×?250 mm analytical column) |
| Column construction | PEEK??with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic. |
| Dimensions | DNAPac PA100 Analytical Column:
2?×?250 mm
DNAPac PA100 Guard Column:
2 × 50 mm
DNAPac PA100 Analytical Column:
4 × 250 mm
DNAPac PA100 Guard Column:
4 × 50 mm
DNAPac PA100 Semipreparative Column:
9 × 250 mm
DNAPac PA100 Semipreparative Column:
22 × 250 mm
DNAPac PA100 Semipreparative Guard Column:
22 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Analytical Columns |
|---|
| DNAPac PA-100 Analytical Column (4 x 250 mm) | 043010 |
| DNAPac PA-100 Semi-Preparative Column (9 x 250 mm) | 043011 |
| DNAPac PA-100 Semi-Preparative Column (22 x 250 mm) | SP2091 |
| FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 150 | SP4008 |
| FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 600 | SP3229 |
| DNA Pac PA-100 Analytical Column (2 x 250 mm) | SP3686 |
| DNAPac PA-100 Column (22 x 50 mm) | SP4513 |
| FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 10 | SP4757 |
| Guard Columns |
|---|
| DNAPac PA-100 Guard Column (2 x 50 mm) | SP4016 |
| DNAPac PA-100 Guard Column (4 x 50 mm) | 043018 |
