SP5732 美國戴安Dionex ProPac SAX and WAX Protein Column 生物色譜柱
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產(chǎn)品名稱: SP5732 美國戴安Dionex ProPac SAX and WAX Protein Column 生物色譜柱
產(chǎn)品型號: SP5732
產(chǎn)品展商: 戴安Dionex
產(chǎn)品文檔: 無相關文檔
簡單介紹
戴安公司生產(chǎn)的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術優(yōu)勢和方法已廣泛地應用于各個領域，其市場占有率達80%。全球與離子色譜相關的文章有90%均出自戴安公司生產(chǎn)的離子色譜儀。
戴安公司的快速溶劑萃取儀15分鐘之內(nèi)僅用15毫升溶劑既可完成對10g樣品的萃取，該技術已在環(huán)境、**、食品和聚合物工業(yè)等領域得到廣泛應用。
SP5732 美國戴安Dionex ProPac SAX and WAX Protein Column 生物色譜柱
的詳細介紹
High-Resolution, Strong and Weak Anion-Exchange Columns for the Separation of Proteins
The ProPac® SAX-10 is a nonporous, strong anion-exchange column (quaternary amine
functional group). The ProPac WAX-10 column is a weak anion exchanger
(tertiary amine functional group). Both are based on a nonporous core
particle that provides pressure stability. A hydrophilic layer prevents
unwanted secondary interactions and a grafted anion exchange surface
provides pH control, selectivity, and fast mass transfer for high
efficiency separation, as well as moderate capacity.
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Unequalled resolution
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High-efficiency peaks with excellent recovery
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Higher capacity than other columns based on nonporous particles
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Useful for characterization and quality control assessment of closely-related protein variants
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Supports separation of proteins that differ by as little as one amino acid residue
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Neutral hydrophilic coat that eliminates protein-resin hydrophobic interactions
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Superior lot-to-lot and column-to-column reproducibility
ProPac
SAX-10 and WAX-10 columns can resolve protein isoforms that differ by
as little as one charged residue. Differences in selectivity between the
WAX-10 and SAX-10 provide flexibility in maximizing resolution of
closely related species.
All ProPac columns are manufactured and
tested under the strictest specifications, resulting in unmatched
column-to-column and lot-to-lot reproducibility. Dionex also offers
these columns as lot select packages, containing three columns from one
lot or three columns from three different lots, to assist quality
control scientists in method validation.
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ProPac SAX-10 Strong Anion-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications
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Maximum operating pressure
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3000 psi (21 MPa)
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Maximum operating conditions
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60 °C
Recommended operating temperature: <40 °C
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Typical operating conditions
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UV detection at 1 mL/min
Recommended flow rate: 1 mL/min
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Mobile phase compatibility
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pH 2–12; 80% compatible with acetonitrile, acetone, methanol.
Compatible with nonionic and zwitterionic detergents.
Incompatible with anionic detergents.
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Resin composition
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10-μm-diameter
core bead (55% cross-linked) grafted with a pellicular layer to which
are attached the quaternary ammonium functional groups.
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Anion-exchange capacity
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< 100 μg protein on-column, depending upon the protein
(4 × 250 mm analytical column)
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Column construction
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PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
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ProPac WAX-10 Weak Anion-Exchange Column for the High-Resolution Analysis of Protein Variants Specifications
|
|
Maximum operating pressure
|
3000 psi (21 MPa)
|
|
Maximum operating temperature
|
60 °C
Recommended operating temperature: <40 °C
|
|
Typical operating conditions
|
UV detection at 1 mL/min
Recommended flow rate: 1 mL/min
|
|
Mobile phase compatibility
|
pH 2–12; 80% compatible with acetonitrile, acetone, methanol.
Compatible with nonionic and switterionic detergents.
Incompatible with anionic detergents.
|
|
Resin composition
|
10-μm-diameter
core bead (55% cross-linked) grafted with a pellicular layer to which
are attached the tertiary amine functional groups.
|
|
Anion-exchange capacity
|
< 100 μg protein on-column, depending upon the protein
(4 × 250 mm analytical column)
|
|
Column construction
|
PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
|
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SAX Analytical Columns
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ProPac SAX-10 Analytical Column (2 x 250 mm)
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063448
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ProPac SAX-10 Analytical Column (4 x 250 mm)
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054997
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ProPac SAX-10HT Analytical Column (4 x 50 mm)
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078990
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ProPac SAX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin)
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SP5729
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ProPac SAX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin)
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SP5730
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ProPac SAX-10 Semi-Preparative Column (9 x 250 mm)
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063703
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ProPac SAX-10 Semi-Preparative Column (22 x 250 mm)
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SP5594
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WAX Analytical Columns
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ProPac WAX-10 Analytical Column (2 x 250 mm)
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063464
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ProPac WAX-10 Analytical Column (4 x 250 mm)
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054999
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ProPac WAX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin)
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SP5731
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ProPac WAX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin)
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SP5732
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ProPac WAX-10 Semi-Preparative Column (9 x 250 mm)
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063707
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ProPac WAX-10 Semi-Preparative Column (22 x 250 mm)
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SP5598
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WAX Guard Columns
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ProPac WAX-10G Guard Column (2 x 50 mm)
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063470
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ProPac WAX-10G Guard Column (4 x 50 mm)
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055150
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SAX Guard Columns
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ProPac SAX-10G Guard Column (2 x 50 mm)
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063454
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ProPac SAX-10G Guard Column (4 x 50 mm)
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054998
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