063274美國戴安Dionex ProPac IMAC Column 生物色譜柱
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產品名稱: 063274美國戴安Dionex ProPac IMAC Column 生物色譜柱
產品型號: 063274
產品展商: 戴安Dionex
產品文檔: 無相關文檔
簡單介紹
戴安公司生產的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術優勢和方法已廣泛地應用于各個領域，其市場占有率達80%。全球與離子色譜相關的文章有90%均出自戴安公司生產的離子色譜儀。
戴安公司的快速溶劑萃取儀15分鐘之內僅用15毫升溶劑既可完成對10g樣品的萃取，該技術已在環境、**、食品和聚合物工業等領域得到廣泛應用。
063274美國戴安Dionex ProPac IMAC Column 生物色譜柱
的詳細介紹
Analytical Column for Immobilized Metal Affinity Chromatography
The ProPac® IMAC-10 is an analytical HPLC column packed with 10 μm, nonporous,
polymeric beads that are coated with a hydrophilic layer, then grafted
with poly(IDA) chains. The poly(IDA) grafts are converted to
metal-containing nanoparticles when the column is charged with metal. It
is these nanoparticles that act as IMAC interaction sites for
individual proteins and provide the ProPac IMAC-10 with its high
resolving power.
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State-of-the-art technology for reusable columns with metal tailored specificity
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Resolve target proteins using a single column in a high resolution gradient run
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Retention control by imidazole concentration or pH gradient
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High purity separations of metal-binding proteins
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Wide range of metal-specific applications
The
ProPac IMAC-10 design maximizes the number of sterically accessible
ligands for binding to proteins, ensuring that the proteins remain
tightly bound during separation. It can be operated between pH 2–12 and
is compatible with commonly used protein purification reagents
(non-ionic detergents, reducing agents). It can be used under native or
denaturing HPLC conditions
The ProPac IMAC-10 is shipped free
from any metal and ready to be charged with the metal of your choice. To
avoid contact of the metal solution with your system, charge the
IMAC-10 with a loading column (an empty column body you fill with your
metal solution, then place between the pump and the IMAC-10 column).
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ProPac IMAC Columns Specifications
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Maximum Operating Pressure
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<3000 psi (21 MPa)
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Temperature Range
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<60oC
Recommended Temperature: Controlled ambient
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Typical Operating Conditions
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<600 psi at 1.0 mL/min (4 × 250mm)
Recommended flow rate: 1.0 mL/min
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Mobile Phase Compatibility
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pH
2–12, Compatible with most reagents used in IMAC protein purifications,
including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic
acid, guanidine, HCl
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Resin Compostion
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10 μm diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts.
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Metal Ion Capacity (μmol Cu)
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>80 mmol Cu (4 × 250 mm analytical column)
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Chelating Metals
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Ni, Fe, Cu, others
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Analytical Column Dimensions
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1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm
2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm
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Loading Column Dimensions
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4 × 50 mm, 9 × 50 mm, 9 × 250 mm
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Analytical Columns
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ProPac IMAC-10 Column (1 x 50 mm)
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063617
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ProPac IMAC-10 Column (2 x 50 mm)
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063272
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ProPac IMAC-10 Column (4 x 50 mm)
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063276
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ProPac IMAC-10 Column (9 x 50 mm)
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063615
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ProPac IMAC-10 Column (2 x 250 mm)
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063274
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ProPac IMAC-10 Column (4 x 250 mm)
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063278
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ProPac IMAC-10 Column (9 x 250 mm)
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063280
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ProPac IMAC-10 Column (22 x 250 mm)
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063282
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Accessories
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IMAC Loading Column (4 x 50 mm)
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063667
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IMAC Loading Column (9 x 50 mm)
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063710
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IMAC Loading Column (9 x 250 mm)
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063718
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