DNAPac PA100 Nucleic Acid Column
For n, n-1 Resolution of Single-Stranded Oligonucleotides
The DNAPac? PA100 is a high-resolution anion-exchange column that provides
single-base resolution. It is stable under denaturing conditions,
rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13
μm pellicular, nonporous polymeric resin with bound quaternary
amine-functionalized MicroBeads?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.
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Capable of n, n-1 resolution for oligonucleotides
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Resolves oligonucleotides with secondary structures
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Compatible with solvent, high pH, and high temperatures
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Analyzes phosphorothioate-based clinical samples
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Provides easy scale-up
The
MicroBead resin used in DNAPac columns provides high-resolution
separations to ensure maximum peak purity, and makes linear scale-up of
your separation easy. For more complex separations, the polymeric
properties of the DNAPac PA100 allows great flexibility to select ideal
conditions, including high pH, high temperature, or chaotropic agents.
Synthetic
oligonucleotides can be screened for production yield and failure
sequences using the DNAPac PA100. Unit-base resolution of synthetic
oligonucleotides to 60 bases and beyond has been demonstrated. The
DNAPac PA100 can even be operated under denaturing conditions. Either
high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be
used to eliminate hydrogen bonding, allowing resolution of problem
sequences such as self-complementary sequences or poly-G stretches.
Separates RNA / Double-Stranded DNA
The
DNAPac PA100 is ideally suited for the purification and analysis of
synthetic RNA. Failure sequences are easily separated from the
full-length product. Double-stranded DNA, such as plasmids, or
restriction fragments are also separated.
Analytical separations
on the 4 mm diameter column can be scaled directly to larger diameter
columns so preparative methods can be conveniently developed using small
samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column
is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 ×
250 mm and 22 × 250 mm column yields essentially identical
chromatography.
