美國戴安Dionex PepSwift Columns 164558 生物色譜柱
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產(chǎn)品名稱: 美國戴安Dionex PepSwift Columns 164558 生物色譜柱
產(chǎn)品型號: 164558
產(chǎn)品展商: 戴安Dionex
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
戴安公司生產(chǎn)的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術(shù)優(yōu)勢和方法已廣泛地應(yīng)用于各個領(lǐng)域，其市場占有率達(dá)80%。全球與離子色譜相關(guān)的文章有90%均出自戴安公司生產(chǎn)的離子色譜儀。
戴安公司的快速溶劑萃取儀15分鐘之內(nèi)僅用15毫升溶劑既可完成對10g樣品的萃取，該技術(shù)已在環(huán)境、**、食品和聚合物工業(yè)等領(lǐng)域得到廣泛應(yīng)用。
美國戴安Dionex PepSwift Columns 164558 生物色譜柱
的詳細(xì)介紹
PepSwift Columns
Monolithic Columns for Fast, High-Resolution Protein and Peptide Analysis
PepSwift
monolithic columns are specially designed for fast separation and
identification of proteins and peptides using nano and capillary LC
coupled to MS. PepSwift columns are based on a polystyrene
divinylbenzene copolymer bed. The bed structure of the column offers a
high-quality alternative to traditional microparticulate sorbents,
providing important advantages for high-sensitivity proteomics
applications.
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Polymeric monolithic stationary phases
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High-speed protein/peptide separations (< 10 min)
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High separation efficiency (> 200.000 plates/m)
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High column-to-column reproducibility
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High sensitivity in LC/MS
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Superior lifetime
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Available in 100 μm, 200 μm and 500 μm i.d
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Available in 5 and 25 cm length (only 100 and 200 μm i.d.)
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nanoViper fitting
PepSwift
Trap columns can be used for preconcentration and desalting of samples
consisting of peptides and proteins without negative impact on the
chromatographic performance or recovery of the compounds. Various
ion-pairing agents can be used in the loading solvent and/or mobile
phases to change the selectivity of the separation or improve the
trapping efficiency.
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PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Specifications
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PepSwift RP
|
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Typical Applications
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Peptides, proteins, and oligonucleotides
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Column Dimensions
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100 μm × 50 mm
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200 μm × 50 mm
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500 μm × 50 mm
|
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Base Material
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Polystyrene/divinyl benzene
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Polystyrene/divinyl benzene
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Polystyrene/divinyl benzene
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Chemistry
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Phenyl
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Phenyl
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Phenyl
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Recommended Flow Rate
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700–1000 nL/min
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2–3 μL/min
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15–25 μL/min
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Temperature
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80 ?C
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80 ?C
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80 ?C
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Maximum Pressure
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4350 psi (30 MPa)
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4350 psi (30 MPa)
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4350 psi (30 MPa)
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Recommended Sample Quantity
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5 ng*
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20 ng*
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0.12 μg*
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Sample Capacity***
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0.2 ng
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0.6 ng
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3.8 ng
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pH Stability
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1–10
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1–10
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1–10
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Solvent Compatibility
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Most common RP
solvents
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Most common RP
solvents
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Most common RP
solvents
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*Tryptic digest of six proteins
**E. coli proteins
***
Dynamic loading capacity is defined as the 10% increase in peak width
measured at half height induced by injected mass of a single peptide
(tryptic peptide of cytochrome c for PepSwift RP) and intact protein
(cytochrome c for ProSwift RP-10R), respectively
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PepSwift and ProSwift Monolith Columns
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PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper
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164584
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PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper
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164584
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PepSwift Monolithic Nano Column, 100 μm i.d. x 25 cm, nanoViper
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164543
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PepSwift Monolithic Capillary Column, 200 μm i.d. × 5 cm
|
161409
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PepSwift Monolithic Capillary Column, 200 μm i.d. x 5 cm, nanoViper
|
164557
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PepSwift Monolithic Capillary Column, 200 μm i.d. x 25 cm, nanoViper
|
164542
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PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm
|
164087
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PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm, nanoViper
|
164585
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ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper
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164586
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ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper
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164586
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PepSwift Trap Columns
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PepSwift Monolithic Trap Column, 200 μm x 5 mm, (set of 2), nanoViper
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164558
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PepSwift Monolithic Trap Column, 200 μm x 5 mm, (set of 2), nanoViper
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164558
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