美國戴安Dionex GlycanPac AXH-1 Column 082468 GlycanPac系列生物色譜柱

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產(chǎn)品名稱: 美國戴安Dionex GlycanPac AXH-1 Column 082468 GlycanPac系列生物色譜柱

產(chǎn)品型號(hào): 082468

產(chǎn)品展商: 戴安Dionex

產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

戴安公司生產(chǎn)的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術(shù)優(yōu)勢和方法已廣泛地應(yīng)用于各個(gè)領(lǐng)域，其市場占有率達(dá)80%。全球與離子色譜相關(guān)的文章有90%均出自戴安公司生產(chǎn)的離子色譜儀。　　戴安公司的快速溶劑萃取儀15分鐘之內(nèi)僅用15毫升溶劑既可完成對10g樣品的萃取，該技術(shù)已在環(huán)境、**、食品和聚合物工業(yè)等領(lǐng)域得到廣泛應(yīng)用。

美國戴安Dionex GlycanPac AXH-1 Column 082468 GlycanPac系列生物色譜柱 的詳細(xì)介紹

GlycanPac AXH-1 Glycan Analysis Column

GlycanPac AXH-1 Columns for the High-Resolution Separation and Analysis of Glycans

The Thermo Scientific^? GlycanPac^? AXH-1 column is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.

The GlycanPac AXH-1 column can be used for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules (e.g., proteins).

Features:

Unique glycan selectivity based on charge, size, and polarity
Excellent resolution for both native and labeled glycans
Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
Compatible with fluorescence and MS detection methods
High chromatographic efficiency and excellent column stability

The GlycanPac AXH-1 column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction liquid chromatography (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans, while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 column provides unparalleled capabilities for glycan separations. Additionally, this column offers charge-based separations for various glycans without discrimination of size and polarity, making it a suitable tool for accurate quantification of glycans which cannot be achieved with other HPLC columns. The GlycanPac AXH-1 column is designed and tested for LC-fluorescence detection and LC-MS applications using volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile.

GlycanPac AXH-1 columns are based on high-purity and spherical silica substrates in both 1.9 and 3 μm sizes for UHPLC and standard HPLC applications, respectively. GlycanPac AXH-1 columns are available in various dimensions for applications with different resolutions, throughput, and instrument requirements.

GlycanPac AXH-1 Column Specifications
Column Dimensions	1.9 μm: 2.1 × 250mm, 2.1 × 150mm, 2.1 ×100mm 3 μm: 4.6 × 150mm, 3.0 × 150mm, 2.1 × 150mm
Bead Size	Spherical 1.9 μm, 3 μm particles
Substrate	High-purity, Porous, Spherical Silica

Functional Group	WAX and HILIC Mixed-Mode
Recommended Flow Rate Range:	1.9 μm: 0.1 to 0.4 mL/min. for: all 2.1 mm columns 3 μm: 0.6 to 1.2 ml/min for 4.6 × 150 mm 0.3 to 0.6 ml/min for 3.0 × 150 mm 0.1 to 0.5 ml/min. for 2.1 × 150 mm
Pressure Limit	1.9 μm: 7,000 psi for 2.1 × 100mm, 10,000 psi for 2.1 ×150mm 15,000 psi for 2.1 × 250mm 3 μm: 6,000 psi for all 3 μm columns
Temperature Range:	Ambient to 60 oC
pH Range	2.0 - 8.0

Analytical Columns
GlycanPac AXH-1, 3 μm, Analytical (4.6x150mm)	082468
GlycanPac AXH-1, 3 μm, Analytical (3.0x150mm)	082469
GlycanPac AXH-1, 3 μm, Analytical (2.1x150mm)	082470
GlycanPac AXH-1, 1.9 μm, Analytical (2.1x250mm)	082521
GlycanPac AXH-1, 1.9 μm, Analytical (2.1x150mm)	082472
GlycanPac AXH-1, 1.9 μm, Analytical (2.1x100mm)	082473
GlycanPac AXH-1, 1.9 μm, Analytical (1.0 × 150 mm, with nanoViper)	164863

Guard Columns
GlycanPac AXH-1, 3 μm, Guard (4.6x10 mm) 2ea (Use Holder V-2)	082474
GlycanPac AXH-1, 3 μm, Guard (3.0x10 mm) 2ea (Use Holder V-2)	082475
GlycanPac AXH-1, 3 μm, Guard (2.1x10 mm) 2ea (Use Holder V-2)	082476
Acclaim SST Guard Cartridge Holder V-2	069580
Acclaim Guard Kit (Holder and coupler) V-2	069707
Guard to Analytical Column Coupler V-2	074188