GlycanPac AXR-1 Columns for the High-Resolution Separation and Analysis of Glycans
The Thermo Scientific? GlycanPac? AXR-1 column is a high-performance, silica-based HPLC column for the
simultaneous separation of glycans by charge, isomerism, and size. It is
designed to provide industry-leading resolution with unique
selectivities for biologically important glycans, either labeled or
native, using either fluorescence and/or mass spectrometry (MS)
detection.
Features:
? Unique glycan selectivity based on charge, branch/linkage isomerism, and size
? Exceptional resolution for either native or derivatized glycans
? Utility for both high-resolution glycan structural characterization and glycan quantification
? Compatibility with both MS and fluorescence detection methods
? Reliable column performance
? Available in HPLC (3 μm) and UHPLC (1.9 μm) formats.
The
GlycanPac AXR-1 column, based on innovative, mixed-mode surface
chemistry, combines both weak anion-exchange (WAX) and reversed-phase
interaction mechanisms to produce exceptional glycan selectivity and
industry-leading resolution. The WAX functionality provides retention of
negatively charged glycans that elute in order of increasing charge,
and the reversed-phase mode facilitates resolution of glycans of the
same charge according to their isomerism and size. These attributes
produce unparalleled glycan separations. The GlycanPac AXR-1 column is
designed for HPLC and UHPLC methods using either fluorescence or MS
detection, and uses volatile aqueous buffers (e.g., ammonium acetate or
ammonium formate) and acetonitrile, presenting the eluting glycans ready
for introduction into MS instruments.
GlycanPac AXR-1 columns are
based on high-purity and spherical silica substrates in both 1.9 and 3
μm sizes for UHPLC and standard HPLC applications, respectively.
GlycanPac AXR-1 columns are available in various dimensions for
applications with different resolutions, throughput, and instrument
requirements.
